Isolation and Characterization of Protease Producing Bacteria from Soil Samples of District Kohat, Pakistan
Authors
Imran Shah
Nasir Azam
Ghias ud Din
Nourin Ali
Waheed ullah
Muhammad Qasim
Aamir Shehzad
Noor Muhammad
Keywords:
Protease,
Bacillus species,
Casein and skim milk agar
Abstract
In this study protease producing bacteria from different regions of Kohat, Khyber Pakhtunkhwa, Pakistan were isolated and screened for protease production on skimmed milk agar and casein agar plates. Out of 100 isolates, 12 were found to be protease producing on skimmed milk/casein agar plates. These isolates were subjected to several biochemical tests and gram staining and were identified to be mainly Bacillus species. Optimum enzymatic activity was found to be at 55ËšC and pH 8-9.SDS-PAGE and Zymographic analysis showed that there is mainly one protease with molecular weight of around 50 kDa.
