Frequent environmental contaminants within microbiology laboratory create not only diagnostic quandaries but also poses major risk for health care workers and patients. Objective of our study was to isolate and identify the common laboratory contaminant bacteria with an ultimate goal to reduce false positive culture reports as well as Laboratory acquired infections. The study was conducted in laboratory of Microbiology Department Kohat University of Science and Technology, Kohat from March 2014 to June 2014. Total 5 samples were collected from different areas of laboratory including table, floor, clothing, air, and incubator. Out of 5 collected samples, total 22 bacterial contaminants were isolated and identified by various biochemical tests. Out of 22 bacterial contaminants, 8 (36.36%) was Staphylococcus epidermis which was the most common contaminants. 7 (31.81%) was Bacillus subtiliswhich was the second most common contaminants. 4 (18.18%) was Staphylococcus aerouswhile 3 (13.63%) was Deptheriodswhich showed minimum bacterial contaminants. Precaution should be taken to get rid of these organisms from laboratory by means of proper laboratory disinfection and sterilization as well as personal hygiene of laboratory workers. From our study we conclude that laboratory can be a potential source of contamination to give false positive results.
