ACE Gene Polymorphism in Hypertension Patients from District Kohat

Abstract

Hypertension, an outstanding danger factor for different cardiovascular, fringe vascular and renal occasions is a significant general wellbeing challenge. Reninangiotensin framework being the most essential pathogenic system of hypertension is interceded by a key segment, the angiotensin converting enzyme. The aim of current study was to know the relationship of hypertensive patients with angiotensin converting enzyme gene polymorphism. Total 65 clinically analyzed hypertensive patients with no related sickness condition and five age and sex coordinated clearly sound controls were included in the current study. Polymerase chain reaction was used for the amplification of intron number 16 of Angiotensin converting enzy megene harboring the 287 bp Alu repeat sequence. Homozygous Deletion/Deletion, Insertion/Insertion and heterozygous Insertion/Deletion were the three possible genotypes which were analyzed. After the amplification of products, 2% agarose gel was used for the separation of products. Statistical Package for the Social Sciences 20 (SPSS 20) was used for the statistical analysis; the P value was found non-significant and to avoid any false positive or false negative significance we cannot conclude any result.